![]() ![]() wt_SRR1582651_1.fastq.gz for the ‘left’ and wt_SRR1582651_2.fastq.gz for the ‘right’ read of the paired end sequences). wt_SRR1582651) contains a pair of fastq files (eg. There are paired-end FASTQ formatted Illlumina read files for each of the two conditions, with three biological replicates for each.Ĭopy the data over to your workspace like so:Įach biological replicate (eg. glabrata in nutrient-rich media (WT), as well as under nitrosative stress (GSNO), in addition to other conditions, but we’ll restrict ourselves to just WT and GSNO conditions for demonstration purposes in this workshop. 2015 This work provides a detailed RNA-Seq-based analysis of the transcriptomic landscape of C. Data Content:įor this course we will be using the data from this paper: Defining the transcriptomic landscape of Candida glabrata by RNA-Seq. Similarly in unix you can type ‘’ and you can continue to type in the rest of the command on the new line in the terminal.įor viewing text files, we’ll use the unix utilities ‘head’ (look at the top few lines), ‘cat’ (print the entire contents of the file), and ‘less’ (interactively page through the file), and for viewing PDF formatted files, we’ll use the ‘xpdf’ viewer utility. Some commands can be fairly long to type in, and so they’ll be more easily displayed in this document, we separate parts of the command with ‘' characters and put the rest of the command on the following line.
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